Diabetes was
induced in Wistar male rats,
with i.v. streptozotocin injections
(55 mg/kg). Animals were orally
treated with microencapsulated
80 mg LZ/kg/day and 80 U bovine
INS/kg/day for 7 weeks, admixing
the test dose to the daily powdered
food. A group of untreated diabetic
rats and a group of normoglycaemic
rats were used as controls.
Glycaemic levels and body weights
were weekly monitored. At 3,
5 and 7 weeks from the diabetes
induction, serum and urine samples
were collected and analyzed.
At the end of the treatment
kidneys were harvested |
After 7
weeks of treatment, diabetic
rats were treated by oral gavage
with 80 U bovine INS/kg/day
microencapsulated (MSLZ+INS).
Serum bovine insulin concentration
was measured in treated and
untreated diabetic groups by
mean of bovine insulin ELISA
test (Mercodia, Uppsala, Sweden),
before treatment and 4 hrs after
treatment.
|
After prolonged treatment, the orally
administered microsystem was able
to deliver INS to the haematic circle,
but serum INS concentration was not
enough to control glycaemia compared
to untreated rats.
A partial glycaemic level reduction
was noted in normoglycaemic rats treated
with a higher INS microencapsulated
dose.
|
Normoglycaemic
Wistar rats were treated by oral gavage
with 150 UI MS INS /kg. Diabetic rats
treated with empty MS were used as
controls groups. Glycaemia levels
were monitored after 30, 60, 120,
180 e 240 (and 300) minutes from the
treatment.
Values were indicated as mean ±
SD. Statistical analysis: Student-Newman-Keuls;*=
p<0.05.
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