Experimental
design
Diabetes was induced in Wistar male
rats, with i.v. streptozotocin injection
(55 mg/kg). Animals were orally treated
with 80 mg LZ/kg/day microencapsulated
for 7 weeks, admixing each dose to the
daily powdered food. A group of untreated
diabetic rats and a group of normoglycaemic
rats were used as controls. Glycaemic
levels and body weights were weekly
monitored.
Analysis on sera samples collected
after 3, 5 and 7 weeks from the beginning
of treatment:
Serum AGE (ELISA test by Mouse MoAb
anti- N?-(carboxymethyl) lysine, Clone
No. NF-1G, CosmoBio Co., Ltd, Tokyo,
Japan ).
Analysis on kidneys harvested and
urine 24-hrs collected after the 7 weeks
from the beginning of treatment:
AGE deposit in kidney (immunoistochemistry);
urinary AGE (test ELISA con Mouse MoAb
anti-
N?-(
carboxymethyl)
lysine).
Statistical analysis: Student-Newman-Keuls.
Data are expressed as mean±SD.
