The CAM of a 12-day-old chick embryo incubated
for 4 days with a gelatin sponge loaded with
(A), (C)
the angiogenic fibroblast growth factor-2
(FGF-2) or with (B),
(D) 240 µM
of NAMI-A. Note in (A)
numerous blood vessels converging like spokes
toward the sponge, whereas in (B)
there are very few vessels around the sponge
or converging toward it. (C)
Histologic section of the sponge shows numerous
vessels (arrows) intermingled in a collagenous
matrix among the trabeculae. (D)
No vessels are detectable. Original magnifications:
A, B,
50x; C, D,
400x.
The
CAM model shows NAMI-A to prevent in vivo
angiogenesis at doses known to have anti-metastasis
activity. In fact, NAMI-A concentration in
the lung of mice bearing pulmonary metastases,
following a treatment cycle (35 mg/kg/day
for six consecutive days) active on metastasis
growth, is in the range 100-300 µM (Sava
et al, 1999b; Sava et al, 1998b; Cocchietto
and Sava, 2000).
